Allergic contact dermatitis: Correlation of in vivo confocal imaging to routine histology☆,☆☆,★
Section snippets
Patients
Five patients, 3 female and 2 male with a clinical history of ACD, were entered after signed informed consent under a Massachusetts General Hospital Institutional Review Board–approved protocol. Patch-testing was performed with specific contact allergens by means of Finn Chambers (Epitest Ltd, Oy, Helsinki) affixed with Scanpor tape (Norgesplaster, Vennesia, Norway). Allergens (balsam of Peru, fragrance mix, and nickel sulfate) were applied to ventral forearm skin for 24 and 48 hours. Contact
Normal skin
In normal skin, CM showed orthokeratotic (no nuclei) and bright stratum corneum at the top of the skin surface (Fig 1, A ).
DISCUSSION
Evaluation of cutaneous delayed hypersensitivity response is of significant interest in dermatology. However, current methods to investigate ACD are limited by the use of indirect techniques that may be inaccurate or insensitive or by the need for multiple invasive biopsies, because it is a dynamic process. Noninvasive imaging tools for the evaluation of contact skin reactions allow the skin to be imaged as many times as required; standard noninvasive imaging tools (eg, magnetic resonance
Acknowledgements
We thank Dr. Aldo González-Serva for critically reviewing the manuscript.
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Supported in part by Lucid Inc.
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Reprint requests: Salvador González, MD, Wellman Laboratories of Photomedicine, BHX-630, Massachusetts General Hospital, 55 Blossom St, Boston, MA 02114.
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