ImmunosuppressantEffect of Rapamycin on Wound Healing: An Experimental Study
Section snippets
Animals
Fourteen male Sprague Dawley rats were obtained from our Laboratory Animal Center. The animals were housed in separate cages in a temperature-controlled room with alternating 12-hour light–dark cycles, and acclimatized for at least 7 days before use. They were fed standard laboratory chow until the night before the operation, and were given water ad libitum. All experimental manipulations were undertaken in accordance with the National Institutes of Health Guide for the Care and Use of
Results
None of the animals in either group had a full-thickness wound dehiscence. Mean RAPA levels were 9.1 ± 0.8 ng/ml. Eosinophil infiltration was significantly higher in the bladder (P = .01), fascia (P = .004), and dermis (P = .01) of the control group. Neutrophil leukocyte infiltration also was significantly high in the bladder (P = .01), fascia (P = .02), and dermis (P = .02) in the control group. The lymphocyte infiltration was similar in each group.
The presence and degree of myofibroblast was
Discussion
RAPA is a potent new immunosuppressive agent that has been shown to significantly reduce the incidence of acute rejection. RAPA had other advantages; for example, unlike calcineurin inhibitors, it is not nephrotoxic and, by decreasing fibrosis, may prevent chronic rejection and stenosis at tracheal anastomoses. Unfortunately, RAPA inhibits the proliferation of fibroblasts; there are concerns that this effect may compromise the healing of certain tissues. Some studies have reported high wound
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