Mechanisms of allergy and clinical immunologyHuman TH17 cells express a functional IL-13 receptor and IL-13 attenuates IL-17A production
Section snippets
Isolation of human T cells
All studies were approved by the Institutional Review Board at Vanderbilt University Medical Center. Human PBMCs were isolated from the buffy coats of healthy donors by using Ficoll-Paque Plus (GE Healthcare, Piscataway, NJ). Naive CD4+ T cells were isolated from the PBMCs by using a naive T-cell isolation kit (Miltenyi Biotec, Auburn, Calif). Briefly, CD4+ T cells were negatively selected by incubating mononuclear cells with biotin-labeled antibodies targeting non-CD4+ cells and CD45R0+ memory
Functional IL-13R is expressed on human TH17 cells
Naive human CD4+ T cells were activated with anti-CD3 and anti-CD28 and polarized to become TH1, TH2, TH17, or iTreg cells. Total cellular RNA was collected 4 days after polarization and analyzed for IL-13Rα1 by means of real-time PCR (Fig 1, A). Flow cytometry was conducted and cells were gated on CD3+CD4+ cells to examine surface expression of IL-13Rα1. IL-13Rα1 expression was significantly increased on TH17 cells both as percentages (Fig 1, B) and total numbers of CD3+CD4+IL-13Rα1+ cells (
Discussion
We have previously shown that IL-13Rα1 is expressed on murine TH17 cells and that IL-13 attenuates IL-17A production from TH17 cells.8 In this study we extend these findings to show for the first time that human TH17 cells express IL-13Rα1 and that TH1, TH2, and iTreg cells do not. This is the first report that IL-13Rα1 is expressed in human T cells, likely because TH17 cells were only recently discovered and IL-13Rα1 was not found in other T-cell lineages. We show IL-13Rα1 expression by means
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Supported by the National Institutes of Health (R01 HL 090664, R01 AI 070672, R01 AI 059108, GM 015431, R21 HL106446, R56AI076411 and F32 HL091653), the Department of Veteran Affairs (1I01BX000624), and Vanderbilt Institute for Clinical and Translational Research (VICTR) grant UL1 RR024975.
Disclosure of potential conflict of interest: G. K. Khurana Hershey and J. K. Kolls have received research support from the National Institutes of Health. R. S. Peebles, Jr, has received research support from the National Institute of Allergy and Infectious Diseases; the National Heart, Lung, and Blood Institute; and the Veterans Administration. The rest of the authors have declared that they have no conflict of interest.