New Directions in Dermatopathology: In Vivo Confocal Microscopy in Clinical Practice
Introduction
Reflectance confocal microscopy (RCM) represents a new imaging tool that enables the identification of cells and tissues with nearly histologic resolution.1, 2, 3 Although several noninvasive tools have been explored to test their potential application in the clinical field, RCM has emerged as a unique instrument because it can visualize the skin tissue with a resolution that is comparable with conventional histopathology. It allows a horizontal scanning of the imaged tissue, with the advantage of exploring a larger field of view compared with vertical sectioning. Moreover, the horizontal plane offers a perfect correlation with clinical and dermoscopic aspects, which is crucial when dealing with skin tumor diagnosis. In this article, we present the main confocal findings and their correlations with histopathology along with a brief description of confocal applications in the clinic arena.
Section snippets
Instruments
The commercially available confocal microscope (VivaScope 1500, Lucid, Rochester, NY) contains a probe (the head of the microscope), which is attached to the skin by using a disposable plastic window, which is in turn taped to a metal ring. A confocal microscope consists of a point source of light, condenser, objective lenses, and a point detector.1, 2 The pinhole collects light emanating only from the in focus plane. The mechanism of bright contrast in RCM is backscattering. In gray-scale
Epidermis
The epidermis can be affected by several injuries that lead to different morphologic changes involving the keratinocytes (KCs) or other cells of the epidermis, such as melanocytes.
The epidermal changes are described as phenomenon per se regardless of their relationship with either inflammatory or skin tumors.
In healthy young skin, the epidermis appears as a multilayer tissue with paradigmatic confocal aspects depending on the skin level.4, 5 The stratum corneum appears as a highly refractive
Skin Tumors
In skin oncology, the goal is to make an early diagnosis and reduce the number of unnecessary biopsies. Because the skin is easy to access, several instruments have been applied in oncology, although the gold standard in clinical practice is considered the combination of clinical inspection and dermoscopy. Dermoscopy is a noninvasive and cheap technique, which has proved to be an essential tool in skin oncology14, 15 and general dermatology.16 RCM represents a second-level examination in
Nonmelanoma skin tumors
Nonmelanocytic tumors include basal cell carcinoma (BCC), actinic keratosis (AK) and squamous cell carcinoma (SCC), to name the most common tumors (Fig. 11). Confocal features of BCC consist of tightly packed aggregates with peripheral palisading and lobulated shape.46, 47, 48, 49 These aggregates are outlined by a dark space (corresponding to mucin) (see Fig. 11A) and are often surrounded by a prominent vascularity. Histopathologically, the aggregates correspond to the basaloid islands. RCM
In Vivo Dermatopathology: Light and Dark
The development of RCM for in vivo examination of the epidermis, papillary, and superficial dermis at a resolution approaching histologic detail has significant implications for clinical care. The possibility of near-histologic resolution at the patient's bedside is fundamental in skin oncology not only for diagnostic purposes but also for nevi follow-up to better understand the dynamic process of growth. In addition, RCM provides an excellent assessment of skin changes after treatment, with
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Segmentation of cellular patterns in confocal images of melanocytic lesions in vivo via a multiscale encoder-decoder network (MED-Net)
2021, Medical Image AnalysisCitation Excerpt :Finally, MED-Net was explicitly designed as a segmentation tool that can be used for other imaging modalities. The multiscale cellular and morphological textural patterns seen in RCM images of melanocytic skin lesions have fundamental underlying similarities to patterns seen in other conditions (e.g. non-melanocytic skin lesions, skin pre-cancers, oral pre-cancers and cancers, benign and inflammatory conditions in skin (Flores et al., 2019; Peterson et al., 2019; Longo et al., 2012)) and with other emerging optical microscopic imaging approaches (optical coherence tomography (OCT), multimodal OCT-and-RCM, and optical coherence microscopy (OCM)) (Schneider et al., 2019; Boone et al., 2015). Thus we also hope that MED-Net will eventually help to drive wider applicability, acceptance and adoption of in vivo optical microscopy in clinical practice.
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2018, JAAD Case ReportsCitation Excerpt :Paradoxically, single-site metastases carry a significantly worse prognosis than multiple-site metastases1 and may be attributed to difficulty in identifying a singular lesion. In vivo reflectance confocal microscopy (RCM) is a novel, noninvasive diagnostic alternative to skin biopsy with comparable insurance reimbursement3 that captures real-time, high-resolution, cellular-level images from the skin surface down to the reticular dermis (up to 300 μm depth).4 This modality forgoes traumatic biopsy and has been used for diagnosis and monitoring of skin cancers and inflammatory dermatoses.5,6
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No conflict of interest to disclose.