Sensory systemComplete overlap of interleukin-31 receptor A and oncostatin M receptor β in the adult dorsal root ganglia with distinct developmental expression patterns
Section snippets
Mice
In the present study, postnatal (0-, 7-, 10-, 12-, and 14-day-old) and adult (6- to 10-week-old) C57BL/6J male mice (Clea Japan, Tokyo, Japan) were used. These mice were kept under a 12-h light/dark cycle with food and water. At all times, the experiments were carried out under the control of the Animal Research Control Committee in accordance with the Guidelines for Animal experiments of Wakayama Medical University, Japanese Government Notification on Feeding and Safekeeping of Animals (No.
Expression of IL-31RA mRNA in CNS and peripheral nervous tissue specimens
We first examined the expression of IL-31RA mRNA in various regions of the CNS, TGs, and DRGs using a northern blot analysis. As shown in Fig. 1A, a single band of 4.8 kb corresponding to IL-31RA mRNA was strongly expressed in DRGs, while IL-31RA mRNA was not detected in TGs and other brain regions, including the cortex, cerebellum, olfactory bulb, hypothalamus, hippocampus and spinal cord. In addition, an RT-PCR analysis revealed that a single band corresponding to IL-31RA mRNA was detected in
Discussion
In the present study, we found that IL-31RA mRNA was highly expressed in the adult DRGs by both northern blot and RT-PCR analyses (Fig. 1, Fig. 5A). In situ hybridization histochemistry revealed that IL-31RA mRNA was expressed in a subset of small-sized DRG neurons. In addition, all the IL-31RA-expressing neurons contained OSMRβ in adult DRGs. These results suggest that a functional receptor for IL-31 exists in a subset of small-sized DRG neurons, which are TRPV1/P2X3/OSMRβ-triple positive (
Acknowledgments
This work was supported by a Research Grant on Priority Areas from Wakayama Medical University.
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