TY - JOUR T1 - Sequential Histological and Immunohistochemical Assessment of Proliferation and Apoptotic Markers During Treatment of Psoriasis With Antitumor Necrosis Factor α (Infliximab) JO - Actas Dermo-Sifiliográficas T2 - AU - Gómez-Mateo,C. AU - Ávalos-Peralta,S.P. AU - Ríos-Martín,J.J. AU - Carrizosa-Esquivel,A.M. AU - González-Cámpora,R. AU - Camacho-Martínez,F. SN - 15782190 M3 - 10.1016/S1578-2190(09)70088-5 DO - 10.1016/S1578-2190(09)70088-5 UR - https://www.actasdermo.org/en-sequential-histological-immunohistochemical-assessment-proliferation-articulo-S1578219009700885 AB - Background and objectivesPsoriasis is an inflammatory skin disease of immunologic nature that is mediated by T-helper-1 cytokines. Clinical response to treatment with antitumor necrosis factor (TNF) a antibodies (infliximab) has been significant; however, the mechanisms for clearance of lesions have not been elucidated. The aim of the present study was to assess variations in the histology and expression of proliferation and apoptotic markers in sequential skin biopsies of patients with psoriasis treated with infliximab. Material and methodsWe studied skin biopsies (of lesioned and healthy skin) from 3 patients with extensive moderate-to-severe psoriasis (mean psoriasis area and severity index [PASI] score, 35) treated with intravenous infliximab infusions (5 mg/kg) at weeks 0, 2, and 6. Biopsies were taken on days 0, 14, and 28, and were processed for conventional histological and immunohistochemical study. The apoptotic markers used were TP53, B-cell lymphoma 2 protein, anticaspase 3, and anticaspase 8. The cell proliferation marker used was Ki67. ResultsTreatment with infliximab was associated with a significant clinical improvement in 3 patients (mean PASI score, 21.6 at 14 days and 13.9 at 6 weeks), which correlated with the progressive disappearance of histological lesions with a decrease in epidermal proliferation. However, apoptosis was not observed, and the samples tested negative for anticaspase antibodies. Expression of TP53 decreased 2 weeks after starting treatment, and was similar to that in normal skin at 28 days. ConclusionsClinical and histological response of psoriasis to infliximab was not associated with a significant increase in the apoptotic markers assessed. ER -